Synthesis, spectral studies, antibacterial and antifungal activity of 2" – methoxy - 4" -[2 - (4' -chlorophenyl)-6-methyl imidazo [1, 2-a] pyridin-3-yl]-6"-aryl nicotinonitrile

Pyridine nucleus plays an important role in medicine, agriculture and industrial chemistry. With a view of biological activities and variety of industrial applications, some new 2" – methoxy - 4" - [2 - ( 4' -chlorophenyl)-6-methyl imidazo [1, 2-a] pyridin-3-yl]-6"-aryl nicotinonitrile( 4a-4l ) has been synthesized. The products have been assayed for their biological activity against Gram +ve, Gram – ve bacteria and fungi. Some of the products showed moderate activity in concentration 50µg/ml. The structures of the products have been elucidated by IR, 1 H-NMR, Mass spectral data, elemental analysis and thin layer chromatography.


INTRODUCTION
Imidazo [1,2-a] pyridines are potential bioactive agents due to their wide spectrum of therapeutic importance. A large number of substituted imidazo [1,2-a]pyridine derivatives are prepared and tested for varieties of biological activities such as, antiallergic 1 , antagonist 2,3 , antifunga 4 , antiepileptic 5 , antibacterial 6 , anticonvulsant 7 , antitubercular 8 , analgesic 9 , insecticidal 10 , antisoriasis 11 , antihypertensive 12 etc. In view of getting to synthesized imidazo [1,2-a] pyridine derivatives and evaluated for their antibacterial antifungal activity. Pyridine, nucleus has been extensively explored for their applications in the field of medicine, agriculture and industrial chemistry. Although many substituted pyridine compounds like other heterocyclic compounds are synthesized with their functional group present from cyclic compounds.
The products (4a-4l) were assigned the IR, 1 H-NMR, Mass spectral data, elemental analysis and TLC. The physical data and antimicrobial activities are represented in Table -I.
All the synthesized compounds (4a-4l) showed moderate to good and remarkable activities with known standard drugs at the same concentration, which is represented in

The physical data and antimicrobial activities of compounds (4a-4l). [Zone of Inhibition in mm]
International Letters of Chemistry, Physics and Astronomy Vol. 49 157

EXPERIMENTAL SECTION
All the melting point was measured by open glass capillary method and are uncorrected. IR absorption spectra ( νmax in cm -1 ) were recorded on a shimadzu IR -435 spectrophotometer using KBr pellet method, 1 H-NMR spectra on Hitachi, R-1200 ( 300-mHz ) spectrometer using DMSO-d6 method, as internal standard ( chemical shift in, δ ppm ) and mass spectra on a Joel 300 ev. The compounds were routinely checked by the TLC using silica gel-G.