Paper Titles

Relative Performance of Students by Gender in Public Examinations (Biology) - A Case of Selected Urban Secondary Schools in Benue State, Nigeria
p.1

Acute Toxicity and Dose Fixation Studies on Chloroxylon swietenia Dc Bark Extracts on Streptozotocin Induced Diabetic Rats
p.8

Callus Induction and Organogenesis in Sugarcane (Saccharum officinarum L.) var 93v297
p.14

In Vitro Seed Germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer
p.23

Ejaculation Malfunctions on the Day of Oocyte Pick up for IVF/ICSI: A Report of Four Cases
p.32

Water Quality Management of the Waters of the Romanian River Hârtibaciu
p.37

Response of Elite Kenyan Finger Millet (Eleusine coracana, L. Gaertn) Genotypes to Ethrel Application
p.43

Contamination and Radionuclides Concentration in Imported Canned Foodstuffs in Baghdad Markets
p.49

Optimization of Banana Peels Hydrolysis for the Production of Bioethanol: Response Surface Methodology
p.53

HomeInternational Letters of Natural SciencesInternational Letters of Natural Sciences Vol. 48In Vitro Seed Germination and Embryo Culture in...

In Vitro Seed Germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

Article Preview

Abstract:

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA₃, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA₃ and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

By email View Pdf

Info:

Periodical:

International Letters of Natural Sciences (Volume 48)

Pages:

23-31

DOI:

https://doi.org/10.56431/p-7rgrk3

Citation:

Cite this paper

Online since:

November 2015

Authors:

S. Kaveri, Rao Srinath*

Keywords:

Embryo Culture, Growth Regulators, H₂SO₄, Mechanical Scarification, N. foetida

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

Share:

Citation:

* - Corresponding Author

[1] Al-Absi K. M. The effect of pre-sowing seed treatments on breaking the dormancy of mahaleb cherries, Prunus mahaleb L. seeds. Seed Sci Technol 38 (2010) 332–340.

DOI: 10.15258/sst.2010.38.2.06

[2] Baskin J. M. and Baskin C. C. Seeds. Ecology, Biogeography and Evolution of Dormancy and germination. Academic press, New York, USA 1988.

DOI: 10.1006/anbo.2000.1238

[3] Bedell P. E. Seed Testing. Seed Science and Technology (Indian Forestry Species), pp. (1998) 218–222

[4] Cetinbas M. and Koyuncu F. Improving germination of Prunu savium L. seeds by gibberellic acid, potassium nitrate and thiourea. Hort Sci 33 (2006) 119–123.

DOI: 10.17221/3750-hortsci

[5] Chauhan J. S., Tomar Y. K., Singh N. I., Ali S. and Debarati. Effect of growth hormones on seed germination and seedling growth of black gram and horse gram. J Am Sci 5 (2009) 79.

[6] Ciddi V. and Shuler M. L. Camptothecin from callus cultures of Nothapodytes foetida. Biotech Lett 22 (2000) 129-132.

[7] Copeland L. and McDonald M. Principles of seed science and technology, fourth ed. Kulwer Academic Publishers Group. MA, USA 2001.

[8] Fathi H. and Jahani U. Review of embryo culture in fruit trees. Annals of Biological Research 3(9) (2013) 4276-4281.

[9] Finkelstein R, Reeves W, Ariizumi T, Steber C Molecular aspects of seed dormancy. Annual Review on Plant Biology 56 (2008) 387-415.

DOI: 10.1146/annurev.arplant.59.032607.092740

[10] Fulzele D. P., Satdive R. K., and Pol B. B. Growth and production of camptothecin by cell suspension cultures of Nothapodytes foetida. Planta Med 67 (2001) 150–152.

DOI: 10.1055/s-2001-11519

[11] Govindachari T. R. and Viswanathan N. Alkaloids of Mappia foetida. Phytochemistry 11 (1972) 3529-3531.

DOI: 10.1016/s0031-9422(00)89852-0

[12] Hidayati S. N., Walck J. L., Merritt D. J., Turner S. R., Turner D. W. and Dixon K. W. Sympatric species of Hibbertia (Dilleniaceae) vary in dormancy break and germination requirements: Implication for classifying morphophysiological dormancy in Mediterranean Biomes. Annals of botany. 109 (2012) 1111-1123.

DOI: 10.1093/aob/mcs034

[13] Holdsworth M. J., Bentsink L. and Soppe W. J. J. Molecular networks regulating Arabidopsis seed maturation, after ripening, dormancy and germination. New Physiologist. 179 (2008) 33-54.

DOI: 10.1111/j.1469-8137.2008.02437.x

[14] Hosseini S. M. N. A. S. R., Savadkoohi S. K. and Ahmadi E. Effect of different seed treatments on dormancy breaking and germination in three species in arid and semi-arid lands. Forest Sci Pract 15 (2013). 130–136.

DOI: 10.1007/s11632-013-0209-7

[15] Jaiswal P. and Chaudhary S. Germination behavior of some trees and grasses of arid lands. Bull Nat Inst Ecol 15 (2005) 201– 205.

[16] Khan M. R. Effect of various treatments on germinability of N. nimmoniana. Bio Sci Res Bull 29(1) (2013) 51-55

[17] Linkies A. and Leubner-Metzger G. Beyong gibberellins and abscisic acid: How ethylene and jasmonates control seed germination. Plant Cell Reports.31 (2012). 253-270.

DOI: 10.1007/s00299-011-1180-1

[18] Liu G. S., Qi D. M. and Zhang W. D. Highly efficient embryo germination in vitro shortens the breeding cycle in Leymus chinensis. In Vitro Cell. Develop. Biol- plant 40 (2004) 321-324.

DOI: 10.1079/ivp2003510

[19] Lorence A. and Nessler C. L. Camptothecin, over four decades of surprising findings. Phytochemistry. 65 (2004) 2735–2749.

DOI: 10.1016/j.phytochem.2004.09.001

[20] Muhammad S. and Amuse N. A. Effects of sulphuric acid and hot water treatments on seed germination of tamarind (Tamarindus indica L). African Journal of Biotechnology. 2 (2003) 276-279.

DOI: 10.5897/ajb2003.000-1056

[21] Muscolo A., Panuccio M. R. and Sidari M. The effect of phenols on respiratory enzymes in seed germination. Respiratory activities during germination of Pinus laricio seeds treated with phenols extracted from different forest soils. Plant Growth Regulation. 35 (2001) 31-35.

DOI: 10.1023/a:1013897321852

[22] Namdeo A. and Sharma A. HPLC analysis of camptothecin content in various parts of Nothapodytes foetida collected on different periods. Asian Pac J Trop Biomed 2 (2012) 389–93.

DOI: 10.1016/s2221-1691(12)60062-8

[23] Padmanabh B. V., Chandrashekhar M., Ramesha B. T., Hombe Gouda H. C. and Gunaga R. P. Pattern accumulation of Camptothecin, an anti-cancer alkaloid in Nothapodytes nimmoniana Graham, in the Western Ghats, India: Implication for identifying high- yielding sources of alkaloid, Curr Sci 90 (2006) 95-100.

DOI: 10.22159//ijpps.2016.v8i9.10751

[24] Pannerselvam K., Bhavanisankar K. and Jayapragasam K. Effect of growth regulators and planting media on rooting of cuttings of N nimmoniana Mabberly. Indian J Plant Physiol 9(3) (2004) 308-312.

[25] Pantazis P. Camptothecin-A promising anti-retroviral drug, J Biomed Sci 3 (1996) 14-19.

[26] Park J., Kim Y. S., Kim S. G., Jung J. H., Woo J. C. and Park C. M. Integration of auxin and salt signals by the NAC transcription factor NTM2 during seed germination in Arabdopsis. Plant Physiology 156 (2011) 537-549.

DOI: 10.1104/pp.111.177071

[27] Rambabu M., Ujjwala D., Ugandhar T., Praveen M., Upender M. and Rama Swamy N. In vitro zygotic embryo culture of an endangered forest tree Givotia rottleriformis and factors affecting its germination and seedling growth. In Vitro Cellular and Developmental biology- plant, 42(5) (2006) 418-421.

DOI: 10.1079/ivp2006804

[28] Roja G. and Heble M. R. The quinoline alkaloids camptothecin and 9-methoxycamptothecin from tissue cultures and mature trees of Nothapodytes nimmoniana. Phytochemistry 36 (1994) 65-66.

DOI: 10.1016/s0031-9422(00)97013-4

[29] Sharma S. N., Puri S. C. and Srivastava T. N. Enhancement of seed germination in N. foetida. J Med Arom Plant Sci 22 (2000) 206-210.

[30] Suhas S., Ramesha B. T., Ravikanth G., Gunaga R. P., Vasudeva R. and Ganeshaiah K. N. Chemical profiling of Nothapodytes nimmoniana populations in the Western Ghats, India for anti-cancer compound, camptothecin. Curr Sci 92 (2007) 1142–1147.

DOI: 10.1007/978-3-540-74603-4_10

[31] Tejavathi D. H., Raveesha H. R. and Shobha K. Evaluation of different treatments to improve the seed germination among the populations of N. foetida (WT) Sleumer. Indian J Fund Appl Life Sci. 1(4) (2011) 187-192.

[32] Urgenc S. and Cepel N. Species Selection for affrestations: Practical Fundamentals of Sowing and Planting. TEMA foundation. Publication no.33, Istanbul 2001.

[33] Ved D. K. Trade in medicinal plants- the state of our ignorance. Amruth, 1 (1997) 2-8.

[34] Wall M. E. and Wani M. C. Camptothecin: Discovery to clinic. Ann. NY Acad. Sci. 803 (1966) 1–12.

[35] Yeung E.C., Thorpe T.A. and Jensen C.J. In vitro fertilization and embryo culture, pp.253-271. In: T.A. Thorpr (ed.). Plant tissue culture: Methods and applications in agriculture. Academic, New York 1981.

DOI: 10.1016/b978-0-12-690680-6.50014-0