Antimicrobial Activity of some Lactobacillus Species against Intestinal Pathogenic Bacteria

Article Preview

Abstract:

Probiotics have antibacterial effects against pathogenic bacteria in the gut while maintaining the balance of intestinal flora such as Lactobacillus. This study aimed to evaluate the antimicrobial activity of four Lactobacillus species against intestinal pathogenic. Four different species of Lactobacillus (Lactobacillus bulgaricus (PTCC 1332), Lactobacillus casei (PTCC 1608), Lactobacillus plantarum (PTCC 1058) and Lactobacillus Fermentum (PTCC 1638)) were experimented to investigate the inhibitory activity against 4 bacterial enteric pathogens (Escherichia coli, Staphylococcus aureus, Shigella dysenteriae and Salmonella paratyphi A) which were separately inoculated in MRS medium (de Man, Rogosa and Sharpe medium) for 48 hours at 37 °C and pH 7. Our results showed that enteropathogens growth was stopped in the presence of all Lactobacillus and inhibition zone was between 12 and 32 millimeter. It can be concluded that these four Lactobacillus strains had potential antimicrobial compounds against human enteric pathogens and should be further studied for their human health benefits.

Info:

* - Corresponding Author

[1] V. Lievin-Le Moal, A.L. Servin, The front line of enteric host defense against unwelcome intrusion of harmful microorganisms: mucins, antimicrobial peptides, and microbiota, Clin. Microbiol. Rev. 19(2) (2006) 315-37.

DOI: 10.1128/cmr.19.2.315-337.2006

Google Scholar

[2] H.L. Turner, J.R. Turner, Good fences make good neighbors: Gastrointestinal mucosal structure, Gut Microbes. 1(1) (2010) 22-29.

DOI: 10.4161/gmic.1.1.11427

Google Scholar

[3] I. Yang, S. Nell, S. Suerbaum, Survival in hostile territory: the microbiota of the stomach, FEMS Microbiology Reviews. 37(5) (2013) 736-761.

DOI: 10.1111/1574-6976.12027

Google Scholar

[4] H. Yazdi et al., The effects of some physicochemical stresses on Escherichia coli O157: H7 as clinical pathogenic bacteria, International Journal of Agriculture & Biology. 18(6) (2016).

DOI: 10.17957/ijab/15.0237

Google Scholar

[5] H. Tebyanian et al., Isolation and identification of Mycoplasma synoviae from suspected ostriches by polymerase chain reaction, in Kerman Province, Iran, Jundishapur Journal of Microbiology. 7(9) (2014).

DOI: 10.5812/jjm.19262

Google Scholar

[6] P. Zarparvar et al., Isolation and identification of culturable halophilic bacteria with producing hydrolytic enzyme from incheh Broun hypersaline wetland in Iran, Cellular and Molecular Biology (Noisy-le-Grand, France). 62(12) (2016) 31-36.

Google Scholar

[7] A. Karami et al., Planarians: an in vivo model for regenerative medicine, International Journal of Stem Cells. 8(2) (2015) 128.

Google Scholar

[8] F. Guarner, J.-R. Malagelada, Gut flora in health and disease, The Lancet. 361(9356) (2003) 512-519.

DOI: 10.1016/s0140-6736(03)12489-0

Google Scholar

[9] R.D. Rolfe, The role of probiotic cultures in the control of gastrointestinal health, The Journal of Nutrition. 130(2) (2000) 396-396.

Google Scholar

[10] Å. Sullivan, C.E. Nord, Probiotics and gastrointestinal diseases, Journal of Internal Medicine. 257(1) (2005) 78-92.

Google Scholar

[11] A.A. Amara, A. Shibl, Role of probiotics in health improvement, infection control and disease treatment and management, Saudi Pharmaceutical Journal. 23(2) (2015) 107-114.

DOI: 10.1016/j.jsps.2013.07.001

Google Scholar

[12] G. Reid, J. Burton, Use of Lactobacillus to prevent infection by pathogenic bacteria, Microbes and Infection. 4(3) (2002) 319-324.

DOI: 10.1016/s1286-4579(02)01544-7

Google Scholar

[13] D.W.K. Acheson, S. Luccioli, Mucosal immune responses, Best Practice & Research Clinical Gastroenterology. 18(2) (2004) 387-404.

DOI: 10.1016/j.bpg.2003.11.002

Google Scholar

[14] A.A. Amara, The inevitability of balanced lives: genes-foods - action-interactions, IIOB Journal. 4(2) (2013) 1-27.

Google Scholar

[15] G. Zanello et al., Saccharomyces boulardii effects on gastrointestinal diseases, Curr. Issues Mol. Biol. 11(1) (2009) 47-58.

Google Scholar

[16] R.J. Boyle, R.M. Robins-Browne, M.L.K. Tang, Probiotic use in clinical practice: what are the risks?, The American Journal of Clinical Nutrition. 83(6) (2006) 1256-1264.

DOI: 10.1093/ajcn/83.6.1256

Google Scholar

[17] S.S. Narayan et al., Probiotics: current trends in the treatment of diarrhoea, Hong Kong medical journal. 16(3) (2010) 213-218.

Google Scholar

[18] V.C. Suvarna, V.U. Boby, Probiotics in human health: A current assessment, Current Science. 88(11) (2005) 1744-1748.

Google Scholar

[19] V. Delcenserie et al., Immunomodulatory effects of probiotics in the intestinal tract, Current Issues in Molecular Biology. 10(1/2) (2008) 37.

Google Scholar

[20] S. Moslehi-Jenabian, L.L. Pedersen, L. Jespersen, Beneficial effects of probiotic and food borne yeasts on human health, Nutrients. 2(4) (2010) 449-473.

DOI: 10.3390/nu2040449

Google Scholar

[21] H. Awad et al., Comparison between killed and living probiotic usage versus placebo for the prevention of necrotizing enterocolitis and sepsis in neonates, Pak. J. Biol. Sci. 13(6) (2010) 253-262.

DOI: 10.3923/pjbs.2010.253.262

Google Scholar

[22] S. Ammor et al., Antibacterial activity of lactic acid bacteria against spoilage and pathogenic bacteria isolated from the same meat small-scale facility: Screening and characterization of the antibacterial compounds, Food Control. 17(6) (2006) 454-461.

DOI: 10.1016/j.foodcont.2005.02.006

Google Scholar

[23] J.-H. Park et al., Antimicrobial effect of lactic acid producing bacteria culture condensate mixture (LCCM) against Salmonella enteritidis, International Journal of Food Microbiology. 101(1) (2005) 111-117.

DOI: 10.1016/j.ijfoodmicro.2004.11.005

Google Scholar

[24] C.N. Jacobsen et al., Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans, Applied and Environmental Microbiology. 65(11) (1999) 4949-4956.

DOI: 10.1128/aem.65.11.4949-4956.1999

Google Scholar

[25] G. Reid et al., Potential uses of probiotics in clinical practice, Clin. Microbiol. Rev. 16(4) (2003) 658-672.

DOI: 10.1128/cmr.16.4.658-672.2003

Google Scholar

[26] O.V. Rybal'chenko, V.M. Bomdarenko, N.B. Verbitskaia, Antagonistic effect of bacteriocinogenic Lactobacillus acidophilus on Klebsiella pneumoniae, Citrobacter freundii and Proteus mirabilis cells, Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii. (7) (2006) 8-11.

Google Scholar

[27] A. Savadogo et al., Antimicrobial activities of lactic acid bacteria strains isolated from Burkina Faso fermented milk, Pakistan Journal of Nutrition. 3(3) (2004) 174-179.

DOI: 10.3923/pjn.2004.174.179

Google Scholar

[28] H. Jamalifar et al., Antimicrobial activity of different Lactobacillus species against multi- drug resistant clinical isolates of Pseudomonas aeruginosa, Iranian Journal of Microbiology. 3(1) (2011) 21-25.

Google Scholar

[29] L. Drago et al., Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin, FEMS Microbiology Letters. 153(2) (1997) 455-463.

DOI: 10.1111/j.1574-6968.1997.tb12610.x

Google Scholar

[30] A. Santos et al., The antimicrobial properties of different strains of Lactobacillus spp. isolated from kefir, Systematic and Applied Microbiology. 26(3) (2003) 434-437.

DOI: 10.1078/072320203322497464

Google Scholar

[31] S. Dasari et al., Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections, Asian Pacific Journal of Tropical Disease. 4(1) (2014) 18-24.

DOI: 10.1016/s2222-1808(14)60307-8

Google Scholar

[32] S. Santosa, E. Farnworth, P.J.H. Jones, Probiotics and their potential health claims, Nutrition Reviews. 64(6) (2006) 265-274.

DOI: 10.1111/j.1753-4887.2006.tb00209.x

Google Scholar

[33] K. Harish, T. Varghese, Probiotics in humans–evidence based review, Calicut Med. J. 4(4) (2006) e3.

Google Scholar

[34] H. Szajewska, J.Z. Mrukowicz, Probiotics in the treatment and prevention of acute infectious diarrhea in infants and children: a systematic review of published randomized, double-blind, placebo-controlled trials, Journal of Pediatric Gastroenterology and Nutrition. 33(2) (2001) S17-S25.

DOI: 10.1097/00005176-200110002-00004

Google Scholar

[35] A. Davoodabadi et al., Antimicrobial activity of Lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic Escherichia coli, Jundishapur J. Microbiol. 8(12) (2015) e27852.

DOI: 10.5812/jjm.27852

Google Scholar

[36] H. Urbancsek et al., Results of a double-blind, randomized study to evaluate the efficacy and safety of Antibiophilus® in patients with radiation-induced diarrhoea, European Journal of Gastroenterology & Hepatology. 13(4) (2001) 391-396.

DOI: 10.1097/00042737-200104000-00015

Google Scholar