News Downloads About Us Contact Us
Paper Titles
Enhancement of Growth and Yield of Arachis hypogeae L. Using Different Biofertilizers
p.1
Bioaccumulation of Cadmium and Lead in the Muscle Tissue of Mullus barbatus in Skikda and Jijel Bays Eastern Algeria
p.10
Surface Water Quality Assessment of Warri Metropolis Using Water Quality Index
p.18
Metagenomics Study of the Microbes in Constructed Wetland System Treating Sewage
p.26
Geographic Pattern as a Determinant Factor of Interlinking Climatic and Hydrological Components of the Natural Resources
p.49

Metagenomics Study of the Microbes in Constructed Wetland System Treating Sewage

Article Preview

Abstract:

Constructed wetlands are ecofriendly, cost effective technology involved in treatment of wastewaters. The goal of this study is focused on characterization of microbial community existing in constructed wetland system planted with Cyperus alternifolius treating sewage contaminated with heavy metals. The characteristics of effluent met the standards of discharge for inland use and irrigation. Microbes in constructed wetland apparently play a pivotal role in the efficiency of system for removal of organics, nutrients, suspended solids and heavy metal. To expose the active players in the lime light, a representative soil sample from the reed bed was collected and characterized for microbial community analysis. Metagenomic studies of the bacterial and fungal flora were identified. Results revealed that the phylum Proteobacteria (38.27%) and Ascomycota (77.47%) dominated in the bacterial and fungal kingdom respectively. However, in the bacterial kingdom at species level major portion remain unclassified except Pseudomonas alcaligenes but in the fungal kingdom at species level only 3.1% remain unclassified. The role of bacteria in wastewater treatment is exemplified in previous reports but the role of fungi in wastewater system needs exploration. However, the findings reveal that the identified microbes might have definitely played a vital role in wastewater treatment. The database available for the identification of bacterial species remain undiscovered for a major portion and requires up gradation. Next generation sequence being a high end technology in microbial ecology decodes the entire community in environmental samples but lack of database limits the identification. Implementation of improvements in the paucity of data bases is essential.

By email View Pdf

Info:

Periodical:

International Letters of Natural Sciences (Volume 74)

Pages:

26-48

DOI:

https://doi.org/10.56431/p-ua15r0

Citation:

Cite this paper

Online since:

March 2019

Authors:

Boopathy Usharani*

Keywords:

Bacteria, Constructed Wetland System, Fungi, Metagenomics, Next Generation Sequence

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

Share:

Citation:

* - Corresponding Author

References

[1] U. Stottmeister et al., Constructed wetlands and their performance for treatment of water contaminated with arsenic and heavy metals, Soil and Water Pollution monitoring protection and remediation, Springer, Dordrecht, 2006.

DOI: 10.1007/978-1-4020-4728-2_27

Google Scholar

[2] A.F. Desta et al., Microbial community structure and diversity in an integrated system of anaerobic-aerobic reactors and a constructed wetland for the treatment of tannery wastewater in Modjo Ethiopia, Plos one. 9 (2014) e115576.

DOI: 10.1371/journal.pone.0115576

Google Scholar

[3] I.N. Balcom et al., Metagenomic analysis of an ecological wastewater treatment plants microbial communities and their potential to metabolize pharmaceuticals, F1000research. 5 (2016) 1-26.

DOI: 10.12688/f1000research.9157.1

Google Scholar

[4] A. Mark Ibekwe, C.M. Grieve, S.R. Lyon, Characterisation of microbial communities and composition in constructed dairy wetland wastewater effluent, Applied Environmental Microbiology. 69 (2003) 95060-95069.

DOI: 10.1128/aem.69.9.5060-5069.2003

Google Scholar

[5] P. Dudhagara et al., Web resources for metagenomic studiesGenomics, Proteomics and Bioinformatics. 13 (2015) 296-303.

Google Scholar

[6] A. Cydzik-Kwiatkowska, M. Zielinska, Bacterial communities in full-scale wastewater treatment systems, World Journal of Microbiology and Biotechnology. 32 (2016) 1-8.

DOI: 10.1007/s11274-016-2012-9

Google Scholar

[7] W. Guan et al., Influence of substrate type on microbial community structure in vertical flow constructed wetland treating polluted river water, Environmental Science Pollution and Research. 22 (2015) 16202-16209.

DOI: 10.1007/s11356-015-5160-9

Google Scholar

[8] P. Arroyo et al., Comparative analysis of the composition of bacterial communities from two constructed wetlands for municipal and swine wastewater treatment, Journal of Water Health. 8 (2010) 147-157.

DOI: 10.2166/wh.2009.123

Google Scholar

[9] S. Takaich et al., Carotenoids of Gemmatimonas aurantiaca (Gemmatimonadetes): identification of a novel carotenoid, deoxyoscillol 2- rhamnoside, and proposed biosynthetic pathway of oscillol 2,2'dirhamnoside, Microbiology. 156 (2009) 757-763.

DOI: 10.1099/mic.0.034249-0

Google Scholar

[10] H. Sun et al., Myriophyllum aquaticum constructed wetland effectively removes nitrogen in swine wastewater, Frontiers in Microbiology. 8 (2017) 1-14.

DOI: 10.3389/fmicb.2017.01932

Google Scholar

[11] DR. Speth et al., Genome based microbial ecology of anammox granules in a full-scale wastewater treatment system, Nature communications. 7 (2016) 11172 -11116

DOI: 10.1038/ncomms11172

Google Scholar

[12] Q. Zhou, T. Chen, S. Han, Characteristics of bacterial communities in Cyanobacteria- blopoming aquaculture wastewater influenced by the phytoremediation with water hyacinth, Water. 9 (2017) 1-11.

DOI: 10.3390/w9120956

Google Scholar

[13] O.V. Tsoy et al., Nitrogen fixation and molecular oxygen:Comparative genomic reconstruction of transcription regulation in alphaproteobacteria, Frontiers in Microbiology. 7 (2016) 1343

DOI: 10.3389/fmicb.2016.01343

Google Scholar

[14] H. Yin et al., An integrated insight into the response of sedimentary microbial communities to heavy metal contamination, Scientific Reports. 5 (2015) 14266.

Google Scholar

[15] P. Swiatczak, A. Cydzik- Kwiatkowska, P. Rusanowska, Microbiota of anaerobic digestors in a full scale wastewater treatment plant, Archives of Environmental Protection. 43 (2018) 53-60.

DOI: 10.1515/aep-2017-0033

Google Scholar

[16] A.M. Kielak et al., The ecology of Acidobacteria: moving beyond genes and genomes, Frontiers in Microbiology. 7 (2016) 744.

Google Scholar

[17] H. Tian et al., Process performance and bacterial community structure under increasing influent disturbances in a membrane aerated biofilm reactors, Journal of Microbiology and Biotechnology. 26 (2016) 373-384.

DOI: 10.4014/jmb.1506.06072

Google Scholar

[18] J.M. Hultman et al., Host range of antibiotic resistance genes in wastewater treatment plant influent and effluent, FEMS Microbiology Ecology. 94 (2018) 1-10.

DOI: 10.1093/femsec/fiy038

Google Scholar

[19] L. Drewniak et al., Physiological and metagenomic analyses of microbial mats involved in self -purification of mine waters contaminated with heavy metals, Frontiers in Microbiology. 7 (2016) 1252.

DOI: 10.3389/fmicb.2016.01252

Google Scholar

[20] B. Zhang et al., Seasonal bacterial community succession in four typical wastewater treatment plants: correlations between core microbes and process performance, Scientific Reports. 8(1) (2018) 4566.

DOI: 10.1038/s41598-018-22683-1

Google Scholar

[21] D.D. Meyer et al., Bacterial communities involved in sulfur transformations in wastewater treatment plants, Applied Microbiology and Biotechnology. 100 (2016) 10125-10135.

DOI: 10.1007/s00253-016-7839-3

Google Scholar

[22] S.J. Mcllroy et al., Genomic and insitu investigations of the novel uncultured Chlorflexi associated with 0092 morphotype filamentous bulking in activated sludge, ISME Journal. 10 (2016) 2223-2234.

DOI: 10.1038/ismej.2016.14

Google Scholar

[23] S. Zielinska et al., First insight into microbial community composition in a phosphogypsum waste heap soil, Acta Biochimica Polonica. 64 (2017) 1-6.

DOI: 10.18388/abp.2017_2297

Google Scholar

[24] M.H. Gerardi, Wastewater bacteria, Wiley Interscience, New Jersy, 2006.

Google Scholar

[25] Q. Ma et al., Identification of the microbial community composition and structure of coal-mine wastewater treatment plants, Microbiology Research. 175 (2015) 1-5.

Google Scholar

[26] T.A. Vishnivetskaya et al., Mercury and other heavy metals influence bacterial community structure in contaminated Tennessee streams, Applied and Environmental Microbiology. 77 (2011) 302-311.

DOI: 10.1128/aem.01715-10

Google Scholar

[27] M. Miransari, Arbuscular mycorrhizal fungi and heavy metal tolerance in plants in: Q.S. Wu (Ed.), Arbuscular Mycorrhizas and stress tolerance of plants, Springer, Singapore, 2017, pp.2659-2668.

DOI: 10.1007/978-981-10-4115-0_7

Google Scholar

[28] M. Naushad Life cycle assessment of wastewater treatment, CRC press, BocaRaton, Florida, 2018.

Google Scholar

[29] D.R. Olicon- Hernandez, J. Gonzalez- Lopez, E. Aranda, Overview on the biochemical potential of filamentous fungi to degrade pharmaceutical compounds, Frontiers in Microbiology. 8 (2017) 1792.

DOI: 10.3389/fmicb.2017.01792

Google Scholar

[30] L. Chen et al., Responses of soil microeukaryotic communities to short-term fumigation-incubation revealed by MiSeq amplicon sequencing, Frontiers in Microbiology. 6 (2015) 1149.

DOI: 10.3389/fmicb.2015.01149

Google Scholar

[31] L.M. Silva- Bedoya et al., Bacterial community analysis of an industrial wastewater treatment plant in Colombia with screening for lipid-degrading microorganisms, Microbiology Research. 192 (2016) 313-325.

DOI: 10.1016/j.micres.2016.08.006

Google Scholar

[32] A. Gonzalez-Martinez et al., Microbial ecology of full-scale wastewater treatment systems in the polar Arctic circle: Archaea, bacteria and fungi, Scientific Reports. 8 (2018) 2208.

DOI: 10.1038/s41598-018-20633-5

Google Scholar

[33] M.M. Mohammadi–Sichani et al., Bioremediation of soil contaminated crude oil by Agaricomycetes, Journal of Environmental Health Science and Engineering. 15 (2017) 1-6.

DOI: 10.1186/s40201-016-0263-x

Google Scholar

[34] M. Fadel et al., Biosorption of manganese from groundwater by biomass of Saccharomyces cerevisiae, HBRC Journal. 13 (2017) 106-113.

DOI: 10.1016/j.hbrcj.2014.12.006

Google Scholar

[35] A. Langarica-Fuentes et al., Fungal succession in an in-vessel composting system characterized using 454 pyrosequencing, FEMS Microbiology. Ecology. 88(2) (2014) 296-308.

DOI: 10.1111/1574-6941.12293

Google Scholar

[36] E.E. Kuramae et al., Structural and functional variation in soil fungal communities associated with litter bags containing maize leaf, FEMS Microbiology. Ecology. 84 (2013) 519-531.

DOI: 10.1111/1574-6941.12080

Google Scholar

[37] AM. Abdel-Azeem et al., Occurrence and diversity of microbiota in heavy metal contaminated sediments of Mediterranean coastal lagoon El-Manzala, Egypt, Mycosphere. 6 (2015) 228-240.

DOI: 10.5943/mycosphere/6/2/12

Google Scholar

[38] X. Wang, G. Li, C.G. Zou Bacteria can mobilize nematode-trapping fungi to kill nematodes. Nature Communications. 5 (2014) 1-9.

Google Scholar

[39] S. Das, H. Ranjan, Dash Handbook of metal-microbe interactions and bioremediation, CRC press, Bocaraton, Florida, 2017.

Google Scholar

[40] VS. Ferreira-Leitao et al., The protagonism of biocatalysis in green chemistry and its environmental benefits, Catalysts. 7 (2017) 1-34.

Google Scholar

[41] S. Sharma, P. Malaviya, Bioremediation of tannery wastewater by chromium resistant novel fungal consortium, Ecological Engineering. 91 (2016) 419-425.

DOI: 10.1016/j.ecoleng.2016.03.005

Google Scholar

[42] K. Widin, B.W. Kennedy, Effect of chemical soil treatment on plant growth, nitrogen fixation, and fungal colonization of Rhizobium nodules of Soybeans', Ecology and Epidemiology. 73 (1983) 429- 430.

DOI: 10.1094/phyto-73-429

Google Scholar

[43] V.D. Jakovljevic, M.M. Vrvic, Potential of pure and mixed cultures of Cladosporium cladosporioides and Geotrichum candidum for application in bioremediation and detergent industry', Saudi Journal of Biological Sciences. 25 (2018) 529-536.

DOI: 10.1016/j.sjbs.2016.01.020

Google Scholar

[44] A. Biedunkiewicz, T. Ozimek, Qualitative and quantitative changes of potentially pathogenic fungi Cunninghamella elegans in a hydrophyte wastewater treatment plant, Polish Journal of Environmental Studies. 18 (2008) 161-166.

Google Scholar

[45] OG. Oladipo et al., Heavy metal torerance traits of filamentous fungi isolated from gold and gemstone mining sites, Brazilian Journal of Microbiology. 4 (2018) 29-37.

DOI: 10.1016/j.bjm.2017.06.003

Google Scholar

[46] A. Bahobil et al., Fungal biopsorption for cadmium and mercury heavy metal ions isolated from some polluted localities in KSA, International Journal of Current Microbiology and Applied Sciences. 6 (2017) 2138-2154.

DOI: 10.20546/ijcmas.2017.606.253

Google Scholar

[47] T.A. Adelani-Akande, O.B. Akpor, B.I. Aderiya, Investigation of the role of selected fungal strains in the removal of phosphate and nitrate in synthetic wastewater' Annual Research & Review in Biology. 4 (2014) 1045-1058.

DOI: 10.9734/arrb/2014/6406

Google Scholar

[48] R. Prasad, Mycoremediation and environmental sustainability: Fungal biology, Springer, Switzerland, 2018.

Google Scholar

[49] V.G. Jogdand et al., Remediation of textile industry wastewater using immobilized Aspergillus terreus, European Journal of Experimental Biology. 2 (2012) 1550-1555.

Google Scholar

© 2025 AOA Academic Open Access Ltd, Cyprus. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied. AcademicOA.com is a registered trademark of AOA Academic Open Access Ltd, Cyprus.