Effect of Nutrient Media and Phytohormones on In Vitro Establishment of Bambusa balcooa. Roxb.

Article Preview

Abstract:

Bambusa balcooa.Roxb. is one of the most commercially important bamboo species. Some varieties of bamboo are being grown in the company’s green house. However this species is very rare and it was not cultured until now due to lack of demand and maintenance problems. In this work B. balcooa ex-plants have been established and propagated by the axillary shoot bud proliferation method. Earlier several works have been done on this plant and a protocol has been. This work emphasizes on effects of phytohormones at different concentrations and combinations upon the novel propagules that develop from the bamboo explants on placing them in multiplication media. The propagules placed in media containing cytokinins BAP (2 mg/L) and Kinetin (1 mg/L) exhibited best results of linear as well as radial growth. The other propagules in media with combinations of BAP, Kinetin and NAA were found to be dried out and did not depict noticeable growth. Comparatively next best growth pattern to the former was observed in control media that lacked phytohormones. This experiment was helpful in estimating the

Info:

Pages:

1-11

Citation:

Online since:

June 2014

Keywords:

Export:

Share:

Citation:

[1] Evans D. A, Sharp W. R, Ammirato P. V., Yamada Y. (1983). Hand book of Plant Cell Culture. Macmillan Publishing Company, New York.

Google Scholar

[2] Pierik R. L. M. (1987). Invitro Culture of Higher Plants. Martinus Nijhoff Publishers, Dordrecht.

Google Scholar

[3] Skoog F., Miller C. O., Soc. Exp. Biol. 11 (1957) 118-131.

Google Scholar

[4] Thorpe T. A. (1981). Plant Tissue culture :Methods and Applications in Agriculture. Academic Press Inc., New York.

Google Scholar

[5] Bhojwani S. S., Razdan M. K. (1990). Plant Tissue Culture: Applications and Limitations. Elsevier Science Publishers, Amsterdam.

Google Scholar

[6] Reinert J., Bajaj P. P. S. (eds.) (1977). Applied and Fundamental Aspects of Plant Cell, Tissue and Organ Culture. Springer-Verlag, Berlin.

DOI: 10.1007/978-3-662-02279-5

Google Scholar

[7] Street H. S. (ed.) (1973). Plant Tissue and Cell Culture. Blackwell Scientific Publication, Oxford. ( Received 19 May 2014; accepted 02 June 2014 )

Google Scholar