Phenolic Compounds and Antioxidant Activity of Rice Straw Extract

Article Preview

Abstract:

Agricultural wastes cause a serious environmental problem in Egypt. Utilization of these wastes by an environmentally friendly way is a very important issue. The objective of this study was to utilize rice straw into high-value products. Antioxidant activity, total soluble phenols, and flavonoids were evaluated in ethyl acetate extract prepared from rice straw after alkaline hydrolysis. Total phenols were found to be 221.6 mg gallic acid equivalents (GAE), while total flavonoids were 4.9 mg rutin equivalents (RE). The extract exhibited strong antioxidant activity measured by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging method and its IC50 value was 0.4 mg/ml. Furthermore, the ethyl acetate extract possessed high antioxidant activity assayed by β-carotene bleaching method and its value of lipid peroxidation inhibition (LPI) was 75.4%. It also exhibited high reducing power and its IC50 value was equal to 0.06 mg/ml. HPLC analysis indicated that this extract contained seven phenolic acids including; protocatechuic, caffeic, syringic, p-coumaric, ferulic, rosmarinic and cinnamic, in addition to two flavonoids; qurecetin and kaempferol. Results indicated that ferulic and p-coumaric acids were the major soluble phenolic acids in rice straw, and their concentrations were 3.9 and 2.9 mg/g DW. It can be summarized that the strong antioxidant activity of ethyl acetate extract, prepared from rice straw, was highly correlated with its high level of phenolic compounds.

Info:

* - Corresponding Author

[1] P. Binod et al., Bioethanol production from rice straw: an overview, Bioresour. Technol. 101(13) (2010) 4767-4774.

Google Scholar

[2] M.A. El-Bendary, Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes, World J. Microbiol. Biotechnol. 26(1) (2010) 153-159.

DOI: 10.1007/s11274-009-0154-8

Google Scholar

[3] S.D. Abou Hussein, O.M. Sawan, The utilization of agricultural waste as one of the environmental issues in Egypt (a case study), J. App. Sci. Res. 6 (2010) 1116-1124.

Google Scholar

[4] O. Akpinar et al., Enzymatic processing and antioxidant activity of agricultural waste autohydrolysis liquors, Bio Resour. 5(2) (2010) 699-711.

Google Scholar

[5] K.A. Jung et al., Pyrolytic production of phenolic compounds from the lignin residues of bioethanol processes, Chem. Eng. J. 259 (2015) 107-116.

DOI: 10.1016/j.cej.2014.07.126

Google Scholar

[6] C. Pouteau et al., Antioxidant properties of lignin in polypropylene, Polymer Degrad. Stab. 81(1) (2003) 9-18.

Google Scholar

[7] C.G. Boeriu et al., Characterisation of structure-dependent functional properties of lignin with infrared spectroscopy, Ind. Crop Prod. 20(2) (2004) 205-218.

Google Scholar

[8] G. Garrote et al., Antioxidant activity of byproducts from the hydrolytic processing of selected lignocellulosic materials, Trends Food Sci. Technol. 15(3) (2004) 191-200.

DOI: 10.1016/j.tifs.2003.09.016

Google Scholar

[9] A. Moure et al., Natural antioxidants from residual sources, Food Chem. 72(2) (2001) 145-171.

Google Scholar

[10] I.S. Mussatto, I.C. Roberto, Optimal experimental condition for hemicellulosic hydrolyzate treatment with activated charcoal for xylitol production, Biotechnol. Prog. 20(1) (2004) 134-139.

DOI: 10.1021/bp034207i

Google Scholar

[11] E. Karimi et al., Identification and quantification of phenolic and flavonoid components in straw and seed husk of some rice varieties (Oryza sativa L.) and their antioxidant properties, J. Sci. Food Agric. 94(11) (2014) 2324-2330.

DOI: 10.1002/jsfa.6567

Google Scholar

[12] D.A.P. de Abreu, K.V. Rodriguez, J.M. Cruz, Extraction, purification and characterization of an antioxidant extract from barley husks and development of an antioxidant active film for food package, Innovative Food Sci. Emerging Technol. 13 (2012) 134-141.

DOI: 10.1016/j.ifset.2011.10.003

Google Scholar

[13] A.A. Elzaawely, S. Tawata, Antioxidant activity of phenolic rich fraction obtained from Convolvulus arvensis L. leaves grown in Egypt, Asian J. Crop Sci. 4 (2012) 32-40.

DOI: 10.3923/ajcs.2012.32.40

Google Scholar

[14] A. Djeridane et al., Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds, Food Chem. 97(4) (2006) 654-660.

DOI: 10.1016/j.foodchem.2005.04.028

Google Scholar

[15] A. A. Elzaawely, T.D. Xuan, S. Tawata, Essential oils, kava pyrones and phenolic compounds from leaves and rhizomes of Alpinia zerumbet (Pers.) B.L. Burtt. & R.M.Sm. and their antioxidant activity, Food Chem. 103(2) (2007) 486-494.

DOI: 10.1016/j.foodchem.2006.08.025

Google Scholar

[16] A.A. Elzaawely, S. Tawata, Antioxidant capacity and phenolic content of Rumex dentatus L. grown in Egypt, J. Crop Sci. Biotechnol. 15 (2012) 59-64.

DOI: 10.1007/s12892-011-0063-x

Google Scholar

[17] A. Yildirim, A. Mavi, A.A. Kara, Antioxidant and antimicrobial activities of Polygonum cognatum Meissn extracts, J. Sci. Food Agric. 83(1) (2003) 64-69.

DOI: 10.1002/jsfa.1288

Google Scholar

[18] Q. Zhang, Z. Zhang, H. Cheung, Antioxidant activity of Rhizoma Smilacis Glabrae extracts and its key constituent-astilbin, Food Chem. 115(1) (2009) 297-303.

DOI: 10.1016/j.foodchem.2008.11.053

Google Scholar

[19] Z.L. Yu et al., Inhibitory effects of Ligustrum robustum (Rxob.) Blume extract on α-amylase and α-glucosidase, J. Funct. Foods. 19 (2015) 204-213.

DOI: 10.1016/j.jff.2015.09.048

Google Scholar

[20] T.K. Hyun et al., Antioxidant, α-glucosidase inhibitory and anti-inflammatory effects of aerial parts extract from Korean crowberry (Empetrum nigrum var. japonicum), Saudi J. Biol. Sci. 23(2) (2016) 181-188.

DOI: 10.1016/j.sjbs.2015.02.008

Google Scholar

[21] M.N. Alam, N.J. Bristi, M. Rafiquzzaman, Review on in vivo and in vitro methods evaluation of antioxidant activity, Saudi Pharm. J. 21 (2013) 143-152.

DOI: 10.1016/j.jsps.2012.05.002

Google Scholar

[22] N. Erkan, G. Ayranci, E. Ayranci, Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmarinic acid and sesamol, Food Chem. 110(1) (2008) 76-82.

DOI: 10.1016/j.foodchem.2008.01.058

Google Scholar

[23] M.S. Blois, Antioxidant determinations by the use of a stable free radical, Nature. 181 (1958) 1199-1200.

DOI: 10.1038/1811199a0

Google Scholar

[24] M. Ali et al., Comparative antioxidant and antimicrobial activities of phenolic compounds extracted from five Hypericum species, Food Technol. Biotechnol. 49 (2011) 205-213.

Google Scholar

[25] G.K. Jayaprakasha, R.P. Singh, K.K. Sakariah, Antioxidant activity of grape seed (Vitis vinifera) extracts on peroxidation models in vitro, Food Chem. 73(3) (2001) 285-290.

DOI: 10.1016/s0308-8146(00)00298-3

Google Scholar

[26] J. Liu et al., The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides, Food Chem. 126(1) (2011) 261-269.

DOI: 10.1016/j.foodchem.2010.11.014

Google Scholar

[27] N. Loganayaki, P. Siddhuraju, S. Manian, Antioxidant activity and free radical scavenging capacity of phenolic extracts from Helicteres isora L. and Ceiba pentandra L., J. Food Sci. Technol. 50(4) (2013) 687-695.

DOI: 10.1007/s13197-011-0389-x

Google Scholar

[28] T. Madhujith, F. Shahidi, Antioxidant potential of barley as affected by alkaline hydrolysis and release of insoluble-bound phenolics, Food Chem. 117(4) (2009) 615-620.

DOI: 10.1016/j.foodchem.2009.04.055

Google Scholar

[29] S.M.A. Salehi et al., Efficient conversion of rice straw to bioethanol using sodium carbonate pretreatment, Energy Fuels. 26(12) (2012) 7354-7361.

DOI: 10.1021/ef301476b

Google Scholar

[30] L. Yang et al., Effects of sodium carbonate pretreatment on the chemical compositions and enzymatic saccharification of rice straw, Bioresour. Technol. 124 (2012) 283-291.

DOI: 10.1016/j.biortech.2012.08.041

Google Scholar

[31] M. Škerget et al., Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities, Food Chem. 89(2) (2005) 191-198.

DOI: 10.1016/j.foodchem.2004.02.025

Google Scholar

[32] P.T. Tuyen et al., Antioxidant capacity and phenolic contents of three Quercus species, Int. Lett. Nat. Sci. 54 (2016) 85-99.

Google Scholar

[33] R.A. Holley, D. Patel, Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials, Food Microbiol. 22(4) (2005) 273-292.

DOI: 10.1016/j.fm.2004.08.006

Google Scholar

[34] H. Zhang, R. Tsao, Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects, Current Opinion Food Sci. 8 (2016) 33-42.

DOI: 10.1016/j.cofs.2016.02.002

Google Scholar

[35] S. Lilitchan et al., Determination of phenolic acid and antioxidant activity from rice straw extracts, Agric. Sci. J. 42 (2011) 377-380.

Google Scholar

[36] A. Abraham et al., Potential of rice straw for bio-refining: An overview, Bioresour. Technol. 215 (2016) 29-36.

Google Scholar

[37] Y. Li et al., Separation and concentration of hydroxycinnamic acids in alkaline hydrolyzate from rice straw by nanofiltration, Sep. Purif. Technol. 149 (2015) 315-321.

DOI: 10.1016/j.seppur.2015.06.006

Google Scholar

[38] T.N. Linh, H. Fujita, A. Sakoda, Release kinetics of esterified p-coumaric acid and ferulic acid from rice straw in mild alkaline solution, Bioresour. Technol. 232 (2017) 192-203.

DOI: 10.1016/j.biortech.2017.02.009

Google Scholar

[39] Y. Xue et al., Effects of different cellulases on the release of phenolic acids from rice straw during saccharification, Bioresour. Technol. 234 (2017) 208-216.

DOI: 10.1016/j.biortech.2017.02.127

Google Scholar