Paper Titles

Melanophores inside Frogs
p.1

Effect of Physical and Chemical Factors in Production of Alkaline Protease Enzyme by Bacillus Strains
p.10

Using Land Cover Change to Predict Forest Degradation Pressure Points, Eastern Mau Forest, Kenya
p.17

Evaluation of Natural Minerals (Zeolite and Bentonite) for Nitrogen Compounds Adsorption in Different Water Temperatures Suitable for Aquaculture
p.34

Comparative Evaluation of the Nutrient Composition and Phytochemical Content of Selected Vegetables Consumed in Nigeria
p.43

HomeInternational Letters of Natural SciencesInternational Letters of Natural Sciences Vol. 71Effect of Physical and Chemical Factors in...

Effect of Physical and Chemical Factors in Production of Alkaline Protease Enzyme by Bacillus Strains

Article Preview

Abstract:

Proteases is family of enzymes and it has crucial role due to their physiological roles and very valuable commercial applications. Alkaline protease are produced by Bacillus species are particular importance because of their thermal stability and stability at different pH values. This study aimed to investigate the effect of physical and chemical factors in production of alkaline protease enzyme fermentation by members of the genus Bacillus. In this study, alkaline protease enzyme production were evaluated in submerged fermentation by Bacillus strains which were isolated from alkaline soils of Guilan province. Factors incubation were optimized such as time, pH, amount of inoculation and ammonium sulfate in alkaline protease enzyme production whit using response surface methodology (RSM) in culture. The maximum enzymatic activity was observed in incubation time of 36 hours, pH=9, inoculation amount of 15% (V) and ammonium sulfate 1.5% (W/V). Factors had significant effect on the production of alkaline protease enzyme such as pH and ammonium sulfate.

By email View Pdf

Info:

Periodical:

International Letters of Natural Sciences (Volume 71)

Pages:

10-16

DOI:

https://doi.org/10.56431/p-1hb5hz

Citation:

Cite this paper

Online since:

September 2018

Authors:

Hamid Tebyanian, Seyed Hanif Mirhosseiny, Afsaneh Bakhtiari, Ali Karami, Sonia Dadseresht, Behzad Otroshi*

Keywords:

Alkaline Protease, Bacillus, RSM

Export:

RIS, BibTeX

Permissions:

Creative Commons CC BY 4.0

Share:

Citation:

* - Corresponding Author

[1] J. Singh, N. Batra, R.C. Sobti, Serine alkaline protease from a newly isolated Bacillus sp. SSR1, Process. Chem. 36(8) (2001) 781-785.

DOI: 10.1016/s0032-9592(00)00275-2

[2] S.-L. Wang et al., Two novel surfactant-stable alkaline proteases from Vibrio fluvialis TKU005 and their applications, Enzyme Microb. Technol. 40(5) (2007) 1213-1220.

DOI: 10.1016/j.enzmictec.2006.09.012

[3] K.M. Sharma et al., Microbial alkaline proteases: Optimization of production parameters and their properties, Genet. Eng. Biotechnol. J. 15(1) (2017) 115-126.

[4] S. Guleria et al., Purification and characterization of detergent stable alkaline protease from Bacillus amyloliquefaciens SP1 isolated from apple rhizosphere, J. Basic Microbiol. 56(2) (2015) 138-152.

DOI: 10.1002/jobm.201500341

[5] M. Baweja et al., An alkaline protease from Bacillus pumilus MP 27: Functional analysis of its binding model toward its applications as detergent additive, Front. Microbiol. 7 (2016) 1195.

DOI: 10.3389/fmicb.2016.01195

[6] H. Tebyanian et al., Antimicrobial activity of some Lactobacillus species against intestinal pathogenic bacteria, International Letters of Natural Sciences. 65 (2017) 10-15.

DOI: 10.56431/p-c620g7

[7] F. Uyar, Z. Baysal, Production and optimization of process parameters for alkaline protease production by a newly isolated Bacillus sp. under solid state fermentation, Process. Chem. 39(12) (2004) 1893-1898.

DOI: 10.1016/j.procbio.2003.09.016

[8] N. Yang et al., Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean wastes, Enzyme Microb Technol. 26(5-6) (2000) 406-413.

DOI: 10.1016/s0141-0229(99)00164-7

[9] H. Yazdi et al., The Effects of some physicochemical stresses on Escherichia coli O157:H7 as clinical pathogenic bacteria, Int. J. Agric. Biol. 18(06) (2016) 1237-1241.

DOI: 10.17957/ijab/15.0237

[10] P. Zarparvar et al., Isolation and identification of culturable halophilic bacteria with producing hydrolytic enzyme from Incheh Broun hypersaline wetland in Iran, Cell. Mol. Biol. (Noisy-le-grand). 62(12) (2016) 31-36.

[11] N. Bhaskar et al., Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities, Bioresour. Technol. 98(14) (2007) 2758-2764.

DOI: 10.1016/j.biortech.2006.09.033

[12] H. Gençkal, Studies on alkaline protease production from Bacillus sp., Master's thesis, İzmir Institute of Technology, 2004.

[13] C.G. Kumar, H. Takagi, Microbial alkaline proteases: from a bioindustrial viewpoint, Biotechnol. Adv. 17(7) (1999) 561-594.

[14] J. Chaloupka, Temperature as a factor regulating the synthesis of microbial enzymes, Microbiol. Sci. 2(3) (1985) 86-90.

[15] S. Ito et al., Enhanced production of extracellular enzymes by mutants of Bacillus that have acquired resistance to vancomycin and ristocetin, Agric. Biol. Chem. 55(9) (1991) 2387-2391.

DOI: 10.1271/bbb1961.55.2387

[16] H. Tebyanian et al., Isolation and identification of Mycoplasma synoviae from suspected ostriches by polymerase chain reaction, in Kerman Province, Iran, Jundishapur J. Microbiol. 7(9) (2014).

DOI: 10.5812/jjm.19262

[17] S. Mehrotra et al., The production of alkaline protease by a Bacillus species isolate, Bioresour. Technol. 67(2) (1999) 201-203.

[18] A.K. Mukherjee, S.K. Rai, A statistical approach for the enhanced production of alkaline protease showing fibrinolytic activity from a newly isolated Gram-negative Bacillus sp. strain AS-S20-I, N. Biotechnol. 28(2) (2011) 182-189.

DOI: 10.1016/j.nbt.2010.11.003

[19] C. Sandhya et al., Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state ferme ntation, Process. Chem. 40(8) (2005) 2689-2694.

DOI: 10.1016/j.procbio.2004.12.001

[20] Q.K. Beg, V. Sahai, R. Gupta, Statistical media optimization and alkaline protease production from Bacillus mojavensis in a bioreactor, Process. Chem. 39(2) (2003) 203-209.

DOI: 10.1016/s0032-9592(03)00064-5

[21] A.K. Mukherjee, H. Adhikari, S.K. Rai, Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: Characterization and application of enzyme in detergent formulation, Biochem. Eng. J. 39(2) (2008) 353-361.

DOI: 10.1016/j.bej.2007.09.017

[22] H. Genckal, C. Tari, Alkaline protease production from alkalophilic Bacillus sp. isolated from natural habitats, Enzyme Microb. Technol. 39(4) (2006) 703-710.

DOI: 10.1016/j.enzmictec.2005.12.004

[23] K.S.B. Naidu, K.L. Devi, Optimization of thermostable alkaline protease production from species of Bacillus using rice bran, Afr. J. Biotechnol. 4(7) (2005) 724-726.

DOI: 10.5897/ajb2005.000-3132

[24] S. Imtiaz, H. Mukhtar, H. Ikram ul, Production of alkaline protease by Bacillus subtilis using solid state fermentation, African Journal of Microbiology Research. 7(16) (2013) 1558-1568.

DOI: 10.5897/ajmr12.1845