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Responses of Flavonoids, Phenolics, and Antioxidant Activity in Rice Seedlings between Japonica and Indica Subtypes to Chilling Stress

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Abstract:

Chilling is one of the major abiotic stress which limites yield and quality of many crops. The seedlings of rice varieties namely Koshihikari (Japonica subtype) chilling tolerant, and the susceptible NMR2 (Indica subtype) were treated at 25/15 °C and 5/4 °C day/night to determine the growth parameters, phenolic contents, and antioxidant activity. It was found that in all treatments, the growth of MNR2 including root and shoot lengths, and leaf and root weights were inhibited at greater levels than Koshihikari. There were seven phenolic acids identified in leaves of Koshihikari including caffeic acid, vanillin, ferulic acid, sinapic acid, benzoic acid, ellagic acid, and cinamic acid, but only benzoic acid and ellagic acid were found in leaves of MNR2. In contrast, only vanillic acid and ellagic acid were observed in roots of Koshihikari, whilst ellagic acid and cinnamic acid were found in roots of MNR2. It was found that rice reduced amount of phenolic acids but promoted quantity of total phenolic content (TPC) and total flavonoid content (TFC) and level of antioxidant activity in chilling stress, although the level of responses varied between Japonica and Indica subtypes. Tolerant rice possessed greater bound flavonoids, phenolics and phenolic acids, but susceptible rice accumulated greater free TPC and TFC in reduced temperature. Findings of this study highlighted that phenolic constituents in bound forms of phenols, polyphenols, and flavonoids may play an active role in rice plants than phenolic acids under chilling stress but need further elaboration.

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Periodical:

International Letters of Natural Sciences (Volume 77)

Pages:

41-50

DOI:

https://doi.org/10.56431/p-3elg24

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Online since:

January 2020

Authors:

Karim Raiuf, Tran Dang Xuan*, Hoang Dung Tran, Naqib Ahmad Fakoori, Tran Dang Khanh, Tran Dang Dat

Keywords:

Antioxidant Activity, Bound Form, Chilling Stress, Free Form, Phenolic Acid, Rice, Total Flavonoid Content, Total Phenolic Content

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Creative Commons CC BY 4.0

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* - Corresponding Author

References

[1] A. Aghaee, et al., Physiological responses of two rice (Oryza sativa L.) genotypes to chilling stress at seedling stage, African Journal of Biotechnology. 10(39) (2011) 7617-7621.

Google Scholar

[2] Q. Zhang, et al., Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci, Rice. 7(1) (2014) 1-12.

DOI: 10.1186/s12284-014-0024-3

Google Scholar

[3] S. C. Xu, et al., Responses of antioxidant enzymes to chilling stress in tobacco seedlings, Agricultural Science in China. 9(11) (2010) 1594-1601.

DOI: 10.1016/s1671-2927(09)60256-x

Google Scholar

[4] S. Zhang, et al., Sex-related differences in morphological, physiological, and ultrastructural responses of Populus cathayana to chilling, Journal of Experimental Botany. 62(2) (2010) 675-686.

DOI: 10.1093/jxb/erq306

Google Scholar

[5] Z. Liu, et al., Water strategy of mycorrhizal rice at low temperature through the regulation of PIP aquaporins with the involvement of trehalose, Applied Soil Ecology. 84 (2014) 185-191.

DOI: 10.1016/j.apsoil.2014.07.010

Google Scholar

[6] A. Ramakrishna, G. A. Ravishankar, Influence of abiotic stress signals on secondary metabolites in plants, Plant Signal Behaviour. 6(11) (2011) 1720-1731.

DOI: 10.4161/psb.6.11.17613

Google Scholar

[7] Y. Sakihama, et al., Plant phenolic antioxidant and prooxidant activities: phenolics-induced oxidative damage mediated by metals in plants, Toxicology. 177(1) (2002) 67-80.

DOI: 10.1016/s0300-483x(02)00196-8

Google Scholar

[8] J.C. Pennycooke, S. Cox, C. Stushnoff, Relationship of cold acclimation, total phenolic content and antioxidant capacity with chilling tolerance in petunia (Petunia x hybrid), Environmental and Experimental Botany. 53(2) (2005) 225-232.

DOI: 10.1016/j.envexpbot.2004.04.002

Google Scholar

[9] N.E. Korres, et al., Temperature and drought impacts on rice production: An agronomic perspective regarding short-and long-term adaptation measures, Water Resources and Rural Development. (9) (2017) 12-27.

DOI: 10.1016/j.wrr.2016.10.001

Google Scholar

[10] L.M. Raboin, et al., Upland rice varieties for smallholder farming in the cold conditions in Madagascar's tropical highlands, Field Crops Research. (169) (2014) 11-20.

DOI: 10.1016/j.fcr.2014.09.006

Google Scholar

[11] J. Suh, et al., Identification and analysis of QTLs controlling cold tolerance at reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa. L), Theory Applied Genetic. 120(5) (2010) 985-995.

DOI: 10.1007/s00122-009-1226-8

Google Scholar

[12] G. Xie, H. Kato, R. Imai, Biochemical identification of the OsMKK6-OsMPK3 signaling pathway for chilling stress tolerance in rice, Biochemical Journal. 443(1) (2012) 95–102.

DOI: 10.1042/bj20111792

Google Scholar

[13] Y.W. Zeng, et al., QTLs of cold tolerance-related traits at the booting stage for NIL-RILs in rice revealed by SSR, Genes Genomics. 31(2) (2009) 143–154.

DOI: 10.1007/bf03191147

Google Scholar

[14] Q.J. Lou, et al., A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.), Euphytica. 158(2) (2007) 87–94.

DOI: 10.1007/s10681-007-9431-5

Google Scholar

[15] K.K. Jena, et al., Identification of cold-tolerant breeding lines by quantitative trait loci associated with cold tolerance in rice, Crop Science. 52(2) (2012) 517–523.

DOI: 10.2135/cropsci2010.12.0733

Google Scholar

[16] F.X. Liu, et al., Identification and mapping of quantitative trait loci controlling cold-tolerance of Chinese common wild rice (O. rufipogon Griff.) at booting to flowering stages, Chinese Science Bulletin. 48(19 (2003) 2068–2071.

DOI: 10.1360/03wc0287

Google Scholar

[17] C. Ye, et al., Cold tolerance in rice varieties at different growth stages, Crop and Pasture Science. 60(4) (2009) 328–338.

DOI: 10.1071/cp09006

Google Scholar

[18] V.C. Andaya, D.J. Mackill, Mapping of QTLs associated with cold tolerance during the vegetative stage in rice, Journal of Experimental Botany. 54(392) (2003) 2579-2585.

DOI: 10.1093/jxb/erg243

Google Scholar

[19] H.H. Hu, et al., Characterization of transcription factor gene SNAC2 conferring cold and salt tolerance in rice, Plant Molecular Biology. 67(2) (2008) 169-181.

DOI: 10.1007/s11103-008-9309-5

Google Scholar

[20] V.C. Andaya, T.H. Tai, Fine mapping of the qCTS12 locus, a major QTL for seedling cold tolerance in rice, Theoritical and Applied Genetic. 113(3) (2006) 467–475.

DOI: 10.1007/s00122-006-0311-5

Google Scholar

[21] Z. Sun, et al., Near-isogenic lines of Japonica rice revealed new QTLs for cold tolerance at booting stage, Agronomy. 9(1) (2019) 40.

DOI: 10.3390/agronomy9010040

Google Scholar

[22] E. Landolt, The family of Lemnaceae. A monographic study. (Vero¨ff. Geobot. Inst. ETH, Zurich), 1987; Vol. 2

Google Scholar

[23] A.A. Elzaawely, T. D. Xuan, S. Tawata, Essential oils, kava pyrones and phenolic compounds from leaves and rhizomes of Alpinia zerumbet (Pers.) B.L. Burtt. & R.M. Sm. and their antioxidant activity, Food Chemistry. 103(2) (2007) 486-494.

DOI: 10.1016/j.foodchem.2006.08.025

Google Scholar

[24] H. Ti, et al., Dynamic changes in the free and bound phenolic compounds and antioxidant activity of brown rice at different germination stages, Food Chemistry. 161 (2014) 337-344.

DOI: 10.1016/j.foodchem.2014.04.024

Google Scholar

[25] T.D. Xuan, et al., Correlation between growth inhibitory exhibition and suspected allelochemicals (phenolic compounds) in the extract of alfalfa (Medicago sativa L.), Plant Production Science. 6(3) (2003) 165-171.

DOI: 10.1626/pps.6.165

Google Scholar

[26] R. Rayee, et al., Imposed water deficit after anthesis for the improvement of macronutrients, quality, phytochemicals, and antioxidants in rice grain, Sustainability 10(12) (2018) 4843.

DOI: 10.3390/su10124843

Google Scholar

[27] R.A. Dixon, et al., The phenylpropanoid pathway and plant defence-a genomics perspective, Molecular Plant Pathology. 3(5) 2002 371-390.

DOI: 10.1046/j.1364-3703.2002.00131.x

Google Scholar

[28] S.Weidner, et al., Phenolic compounds and properties of antioxidants in grapevine roots [Vitis vinifera L.] under drought stress followed by recovery, Acta Societatis Botanicorum Poloniae. 78(2) (2009) 97-103.

DOI: 10.5586/asbp.2009.013

Google Scholar

[29] S. Swigonska, et al., Influence of abiotic stress during soybean germination followed by recovery on the phenolic compounds of radicles and their antioxidant capacity, Acta Societatis Botanicorum Poloniae. 83(3) (2014) 209–218.

DOI: 10.5586/asbp.2014.026

Google Scholar

[30] M.M. Posmyk, et al., Antioxidant enzymes and isoflavonoids in chilled soybean (Glycine max L.) Merr.) seedlings, Journal of Plant Physiology. 162(4) (2005) 403-412.

DOI: 10.1016/j.jplph.2004.08.004

Google Scholar

[31] M.K. Zainol, et al., Antioxidative activity and total phenolic compounds of leaf, root and petiole of four accessions of Centella asiatica (L.), Urban Food Chemistry. 81(4) (2003) 575-581.

DOI: 10.1016/s0308-8146(02)00498-3

Google Scholar

[32] M.M. Oh, H.N. Trick, C.B. Rajashekar, Secondary metabolism and antioxidants are involved in environmental adaptation and stress tolerance in lettuce, Journal of Plant Physiology. 166(2) (2009) 180-191.

DOI: 10.1016/j.jplph.2008.04.015

Google Scholar

[33] D. Mittal, DA. Madhyastha, A. Grover, Genome-wide transcriptional profiles during temperature and oxidative stress reveal coordinated expression patterns and overlapping regulations in rice, Plos One. 7(7) (2012) e40899.

DOI: 10.1371/journal.pone.0040899

Google Scholar

[34] N. Murata, The mechanism of photoinhibition in vivo: Revaluation of the roles of catalase, tocopherol, non-photochemical quenching, and electron transport, Biochimica et Biophysica Acta. 1817(8) (2012) 1127-1133.

DOI: 10.1016/j.bbabio.2012.02.020

Google Scholar

[35] I. Fridovich, The biology of oxygen radicals, Science. 201(4359) (1978) 875-880.

Google Scholar

[36] T.D. Xuan, D.T. Khang, Effects of exogenous application of protocatechuic acid and vanillic acid to chlorophylls, phenolics and antioxidant enzymes of rice (Oryza sativa L.) in submergence, Molecules. 23(3) (2018) 620.

DOI: 10.3390/molecules23030620

Google Scholar

[37] N.T. Quan, et al., Involvement of secondary metabolites in response to drought stress of rice (Oryza sativa L.), Agriculture. 6(2) (2016) 23.

Google Scholar

[38] T.D. Xuan, et al., Allelopathic momilactones A and B are implied in rice drought and salinity tolerance, not weed resistance. Agronomy for Sustainable Development. 36(3) (2016) 52.

DOI: 10.1007/s13593-016-0383-9

Google Scholar

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