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HomeInternational Letters of Natural SciencesInternational Letters of Natural Sciences Vol. 80Evaluation of Genetic Diversity and Identification...

Evaluation of Genetic Diversity and Identification of Huperzia Species Collected in Some Different Areas in Vietnam by Molecular Markers

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Abstract:

The objective of this study was to evaluate genetic diversity of 35 Huperzia samples collected from the different ecological areas in Vietnam by using rbcL markers. The results revealed that the samples were genetically diverse in high genetic similarity which ranged from 98.0 to 99.0%, respectively. Based on the Genbank data in comparison, it was ascertained that only one sample (BK3) is a member of H.phlegmarioides group, and others belonged H. squarrosa group as divided into two main groups by phylogenetic tree analyses. It suggests that some Huperzia samples may plausibly descend from the same origin, evolution and arising relations. This study has provided useful information for further identifying the high-quality genetic plant sources for propagation, development and conservation of the high-valued medicinal materials of Huperzia plants in this country.

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International Letters of Natural Sciences (Volume 80)

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13-23

DOI:

https://doi.org/10.56431/p-mr77lk

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Online since:

December 2020

Authors:

Tran Thi Thu Ha, Tran Dang Khanh, Khuat Huu Trung*

Keywords:

Gene Sequence, Huperzia, ITS, Lycopodiaceae Family, Species

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[1] Singh, H. B. and K. Singh. (2010). Huperzia serrata: a promising medicinal pteridophyte from North East India. NeBIO, 1: 27-34.

[2] Maridass M. and G. Raju (2009). Investigation of Phytochemical and Antimicrobial Activity of Huberzia Species. Pharmacol, 3: 688-692.

[3] Xuyen, D.T. (2013), Current status of plant defects in Kon Ka Kinh National Park, Gia Lai Province, 5th National Scientific Conference on Ecology and Biological Resources.

[4] Yumkham, S.D., and P.K. Singh, (2011). Huperzia squarrose (G.Forst.) Trev. (Lycopodiaceae) in Manipur: Taxonomy and biological aspects. Taiwania, 56(2): 157-164.

[5] Sahidan N., C. Y., Choo Latiff A., R. Jaman. (2012). Variations of huperzine A content in Lycopodiaceae species from tropics. Chin. J. Nat. Med. 10, 125-128.

DOI: 10.3724/sp.j.1009.2012.00125

[6] Nilsu, T., W. Thaisaeng, W. Thamnarak, C. Eurtivong, A. Jumraksa, S. Thorroad, N. Khunnawutmanotham, S. Ruchirawat, N. Thasana. (2018). Three Lycopodium alkaloids from Thai club mosses. Phytochem, 156: 83-88.

DOI: 10.1016/j.phytochem.2018.09.001

[7] Ngoc, V.T., P.T. Hanh, L.T.L. Anh, N.T. Dat, L.T.B. Thuy. (2016). Qualification and quantification of Huperzine a from Huperia serrata in Dalat, Lamdong province. J.Biotech, 14(3): 473-478.

DOI: 10.15625/1811-4989/14/3/9861

[8] Nguyen, H.T., H.T. Doan, D.V. Ho, K.T. Pham, A. Raal, H. Morita. (2018). Huperphegmines A and B, two novel Lycopodium alkaloids with an unprecedented sketon from Huperzia phlegmaria, and their acetylchlinesterase inhibitory activities. Fitoterapia, 129:267-271.

DOI: 10.1016/j.fitote.2018.07.016

[9] Jaswinder, K., S. Rajmeet, S. Gurinder, K. Harpreet, K. Jasvir, K.Manpreet, S. Parminder, K. Jaspreet. (2016). A systematic review on Huperzia serrata. Inter. J.Pharmaco. Phytochem. Res, 8 (8): 1250-1255.

[10] Wang, Z.F., and X.C. Tang. (2007). Huperzine A protects C6 rat glioma cells against oxygen-glucose deprivationinduced injury. FEBS Lett, 581:596–602.

DOI: 10.1016/j.febslet.2007.01.016

[11] Thorroad, S., P. Worawittayanont, N.Khunnawutmantham, N.Chimnoi, A.Jumruksa, S.Ruchirawat, N.Thasana. (2014).Three new Lycopodium alkaloids from Huperzia carinata and Huperzia squarrosa. Tetrahedron,70:8017-8022.

DOI: 10.1016/j.tet.2014.08.042

[12] Nguyen N. C., and C. L. Tran. (2014). Isolation of huperzine A from Huperzia squarrosa (Forst.) Trevis., Lycopodiaceae, J.Med. Mat, 19 (1):22-27.

[13] Zhao X. M., Z. Q. Wang, S. H. Shu, W. J. Wang, H. J. Xu, Y. J. Ahn, M. Wang , X. Hu. (2013). Ethanol and methanol can improve Huperzine A production from Endophytic colletotrichum gloeosporioides. PLoS One 8:4, e61777.

DOI: 10.1371/journal.pone.0061777

[14] Yuan, Q.J., B.Zhang, D.Jiang, W.J. Zhang, T.Y. Lin, N.H. Wang, S.J. Chiou, L.Q. Huang. (2015). Identification of species and materia medica within Angelica L. (Umbelliferae) based on phylogeny inferred from DNA barcodes. Mol.Ecol.Resour. 15(2):358-371.

DOI: 10.1111/1755-0998.12296

[15] Trung, K.H., T.D. Khanh, L.H. Ham, T.D. Duong, N.T. Khoa. (2013). Molecular phylogeny of the endangered Vietnamese Paphiopedilum species based on the internal transcribed spacer of the nuclear ribosomal DNA. Adv.Stud.Biol, 5(7):337-346.

DOI: 10.12988/asb.2013.3315

[16] Parveen, I., H.K. Singh, S.Raghuvanshi, U.C. Pradhan, S.B. Babbar. (2012). DNA barcoding of endangered Indian Paphiopedilum species. Mol.Ecol.Resour, 12(1):82-90.

DOI: 10.1111/j.1755-0998.2011.03071.x

[17] Kress, W.J., and D.Erickson. (2007). A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. Plos One,6: e580.

DOI: 10.1371/journal.pone.0000508

[18] Benniamin, A., V. Irudayaraj and V. S. Manickam (2008). How to identify rare and endangered ferns and fern allies. EthnoBot. Leaflts. 12: 108-117.

[19] Doyle, J.J., J.L. Doyle. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bul, 19:11-15.

[20] Sambrook, J. and D.W. Russell. (2001) Molecular cloning: a laboratory manual. Vol. 2, 3rd edn. Cold Spring Harbor Laboratory Press, New York.

[21] Hasebe, M., T. Omori, M. Nakazawa, T. Sano, K. Iwatsuki. (1994). rbcL gene sequencing provide evidence for the evolutionary lineages of leptosporangiate ferns. Proc Nat Acad Sci USA, 91(12): 5730-5734.

DOI: 10.1073/pnas.91.12.5730

[22] Sundari, N. and N.Papuangan. (2019). Amplication and analysis of rbcL (Ribulose-1,5-Bisphosphate carboxylase) of clove in Ternate island. Inter. Confer. Life. Sci. Tech, 276, 01206.

DOI: 10.1088/1755-1315/276/1/012061

[23] Nam, M, and O.M. Lee (2001). A comparative study of the morphological characters and sequence data of rbcL gene in Cosmarium species. Algae, 16(4): 349-361.

[24] Rajaram, M.C, C.S.Y. Yong, J.A. Gansau, R. Go. (2019). DNA barcoding of endangered Paphiopedilum species (Orchidaceae) of Peninsular Malaysia. Phytotaxa, 387(2): 094-104.

DOI: 10.11646/phytotaxa.387.2.2

[25] Gielly, L, and P. Taberlet, (1994). The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences. Mol. Biol. Evol, 11: 769-777.

DOI: 10.1093/oxfordjournals.molbev.a040157

[26] Dubuisson, J.Y. (1994). rbcL sequences: a promising tool for the molecular systematics of the fern genus Trichomanes (Hymenophyllaceae)?. Mol Phylog Evol 8: 128-138.

DOI: 10.1006/mpev.1997.0414

[27] Fazekas, A.J., Burgess, P.R. Kesanakurti, S.W. Graham, S.G. Newmaster, B.C.K.S. Husband, D.M. Percy, M. Hajibabaei, S.C.H. Barret, (2008). Multiple multilocus DNA barcodes from the plastid genome discriminate plant species. Plos One, 3, e2802.

DOI: 10.1371/journal.pone.0002802

[28] Kress, W.J., K.J. Wurdack, E.A. Zimmer, L.A. Weigt, D.H. Janzen. (2005). Use of DNA barcodes to identify flowering plants. Proc.Nat.Acad. Sci USA, 102: 8369-8374.

DOI: 10.1073/pnas.0503123102

[29] Kesanakurti, P.R., A.J. Fazekas. K.S. Burgess, D.M. Percy, S.G. Newmaster, S.W. Graham, S.C. Barrett, M. Hajibabaei, B.C. Husband. (2011). Spatial patterns of plant diversity below-ground as revealed by DNA barcoding. Mol Ecol, 20:1289-1302.

DOI: 10.1111/j.1365-294x.2010.04989.x

[30] Chase, M.W., N. Salamin, M. Wilkinson, J.M. Dunwell, R.P. Kesana-kurthi, N.Haidar, V. Savolainen. (2005). Land plants and DNA barcodes: short-term and long-term goals. Philos.Trans. R. Soc. Lond. B Biol. Sci.360: 1889–1895. PMID:16214746.

DOI: 10.1098/rstb.2005.1720

[31] Newmanster, S.G., A.J. Fazekas, S. Ragupathy. (2006). DNA barcoding in land plants: evaluation of rbcL in a multigene tiered approach. Ca.J.Bot. 84: 335-341.

DOI: 10.1139/b06-047

[32] Chen, T., X.Chao, L.Lei, C.Li, Y.Zhang, S.Zhou. (2016). Barcoding the kingdom plantae: new PCR primers for ITS regions of plants with improved universality and specificity. Mol.Ecol.Resour,16:138-149.

DOI: 10.1111/1755-0998.12438

[33] Kress, W.J., F.A. Erickson, F.A. Jones, N.G. Swenson, R. Perez, O. Sanjur, E. Bermingham. (2009). Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proc.Nat. Acad. Sci USA, 106:18621-18626.

DOI: 10.1073/pnas.0909820106

[34] Bell, K., V.M., Loeffler, B.J. Brosi. (2017). An rbcL reference library to aid in the identification of plant species mixtures by DNA metabarcoding. App.Plant Sci, 5(3)

DOI: 10.3732/apps.1600110