Effect of Lactic Acid on α-Amylase Activity and Phytic Acid Content in Germination of Rice (Oryza sativa L.)

Article Preview

Abstract:

Lactic acid has known as a one of compounds to cause cellular harm in waterlogged tissue through the process of cytoplasmic acidosis. The effects of lactic acid on α-amylase activity and phytic acid content using an assay for high phosphate in germination stage of rice were evaluated. It is showed that lactic acid inhibited rice germination at every treated dose. The reduction of α-amylase content attributed to lactic acid at 24 h after germination of rice seeds was observed. The analysis of phosphate concentration at 48 h after treatment noted that although the content of phytic acid was reduced in Japonica varieties, but in contrast its amount was enhanced in Indica cultivars. The findings highlighted the positive effects of lactic acid on α-amylase activity and phytic acid content and suggested that this compound may play a potent role as a germinated regulator in rice.

Info:

Pages:

9-15

Citation:

Online since:

February 2018

Export:

Share:

Citation:

* - Corresponding Author

[1] S.S. Kulkarni, P.D. Chavan, Influence of lactic acid on seed germination of finger millet (Eleusine coracana Gaertn.), International Journal of Applied Biology and Pharmaceutical Technology. 3 (2012) 242-247.

Google Scholar

[2] P.W. Hochachaka, T.P. Mommsen, Protons and anaerobiosis, Science. 219(4591) (1983) 1391-1397.

DOI: 10.1126/science.6298937

Google Scholar

[3] K.A. Howell, A.H. Millar, J. Whelan, Ordered assembly of mitochondria during rice germination begins with promitochondrial structures rich in components of the protein import apparatus, Plant Molecular Biology. 60(2) (2006) 201-223.

DOI: 10.1007/s11103-005-3688-7

Google Scholar

[4] G.B. Fincher, Molecular and cell biology associated with endosperm mobilization in germinating cereal grains, Annual Review of Plant Physiology and Plant Molecular Biology. 40(1) (1989) 305-346.

DOI: 10.1146/annurev.pp.40.060189.001513

Google Scholar

[5] T. Akazawa, I. Hara-Mishimura, Topographic aspects of biosynthesis, extracellular section and intracellular storage of proteins in plant cells, Annual Review of Plant Physiology. 36(1) (1985) 441–472.

DOI: 10.1146/annurev.pp.36.060185.002301

Google Scholar

[6] E. Beck, P. Ziegler, Biosynthesis and degradation of starch in higher plants, Annual Review of Plant Physiology and Plant Molecular Biology. 40(1) (1989) 95–117.

DOI: 10.1146/annurev.pp.40.060189.000523

Google Scholar

[7] M.A. Zayed, I.M. Zeid, Effect of water and salt stress on growth, chlorophyll, mineral ions and organic solutes contents on enzyme activity in mug bean seedlings, Biologia Plantarum. 40(3) (1997) 351-356.

DOI: 10.1023/a:1001057728794

Google Scholar

[8] R.N. Trethewey, A.M. Smith, Starch mobilization in leaves, in: R.C. Leegood, T.D. Sharkey, S. von Caemmerer (Eds.), Photosynthesis. Advances in Photosynthesis and Respiration, vol. 9, Springer, Dordrecht, 2000, pp.205-231.

DOI: 10.1007/0-306-48137-5_9

Google Scholar

[9] M. Saeed, S.H. Duke, Amylase in pea tissues with reduce chloroplast density and/or function, Plant Physiology. 94(4) (1990) 1813-1819.

DOI: 10.1104/pp.94.4.1813

Google Scholar

[10] T. Heits et al., Two apoplastic α-amylase are induced in tobacco by virus infection, Plant Physiology. 97(2) (1991) 651-656.

Google Scholar

[11] M.Y. Ashraf et al., Alpha-amylase, protease activities and associated changes under water stress condition in wheat seedling, Pakistan Journal of Scientific and Industrial Research. 38 (1995) 430-434.

Google Scholar

[12] P.D. Commuri, S.H. Duke, Apoplastic α-amylase in pea is enhanced by heat stress, Plant Cell Physiology. 38(5) (1997) 625-630.

DOI: 10.1093/oxfordjournals.pcp.a029213

Google Scholar

[13] J. Shi et al., The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds, The Plant Journal. 42(5) (2005) 708-719.

DOI: 10.1111/j.1365-313x.2005.02412.x

Google Scholar

[14] A. Beleia, L.T. Thu Thao, E. I. Ida, Lowering phytic phosphorus by hydration of soybean, Journal of Food Science. 58(2) (1993) 375-377.

DOI: 10.1111/j.1365-2621.1993.tb04278.x

Google Scholar

[15] E. Loreti et al., Repression of α-amylase activity by anoxia in grains of barley is independent of ethanol toxicity or action of abscisic acid, Plant Biology. 4(2) (2002) 266-272.

DOI: 10.1055/s-2002-25739

Google Scholar

[16] M. Haque, et al., Yield contributing characters effect of submerged water levels of boro rice (Oryza sativa L.), Scientia Agricultural. 9(1) (2015) 23-29.

DOI: 10.15192/pscp.sa.2015.9.1.2329

Google Scholar

[17] P. Bernfeld, Amylases, α and β, in: Methods in enzymology, S.P. Colowick, N.D. Kaplan, (Eds.), Vol. 1, Academic Press, New York, 1955, pp.149-150.

Google Scholar

[18] P.S. Chen, T.Y. Toribara, H. Warner, Microdeteminations of phosphorous, Analytical Chemical. 28 (1956) 1756-1758.

Google Scholar

[19] S.S. Kulkarni, P.D. Chavan, Study of some aspects of anaerobic metabolism in roots of finger millet and rice plants subjected to water logging stress, International Journal of Botany. 9(2) (2013) 80-85.

DOI: 10.3923/ijb.2013.80.85

Google Scholar

[20] A.B. Khattak et al., Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicer arietinum L.) sprouts, Food Chemistry. 104(3) (2007) 1074-1079.

DOI: 10.1016/j.foodchem.2007.01.022

Google Scholar